1. What is the primary purpose of recombinant DNA technology in biotechnology?

a) To enhance photosynthesis in plants
b) To develop vaccines
c) To introduce foreign genes into an organism
d) To study natural ecosystems

2. Which organism is commonly used as a vector in gene cloning?

a) Mice
b) Escherichia coli (E. coli)
c) Yeast
d) Fruit flies

3. Which of the following techniques is used to amplify DNA sequences?

a) Polymerase Chain Reaction (PCR)
b) Gel Electrophoresis
c) DNA Sequencing
d) Southern Blotting

4. Golden Rice is genetically modified to produce which nutrient?

a) Vitamin C
b) Vitamin A
c) Iron
d) Calcium

5. What is the full form of CRISPR, a revolutionary gene-editing tool?

a) Clustered Regularly Interspaced Short Palindromic Repeats
b) Coded Repeated Interspaced Sequence Palindromes
c) Continuous Random Inserted Sequence Programming
d) Clustered Repetitive Intron Sequence Regulation

6. Bt cotton is genetically engineered to be resistant to:

a) Viruses
b) Insects
c) Drought
d) Herbicides

7. Which of the following is used to cut DNA into smaller fragments in genetic engineering?

a) DNA Ligase
b) Restriction Enzymes
c) RNA Polymerase
d) Helicase

8. Which of the following biotechnology techniques is used for DNA fingerprinting?

a) PCR
b) RFLP (Restriction Fragment Length Polymorphism)
c) Gene Therapy
d) Cloning

9.In the production of insulin using biotechnology, which organism is most commonly used?

a) Yeast
b) Bacteria
c) Plants
d) Mice

10. Which type of biotechnological application involves replacing faulty genes with functional ones?

a) Genetic engineering
b) Gene therapy
c) DNA cloning
d) Bioinformatics